Deoxyribonucleic acid (DNA) carries all of the genetic information of the cell. Ribonucleotide reductase (RNR) provides balanced pools of all the four dNTPs, the building blocks of DNA. These foundations are required during DNA synthesis and repair. Failing within the power over the dNTP levels and/or their relative amounts results in cell death or genetic abnormalities. Due to its central role in dNTP metabolism, RNR is extremely regulated on multiple levels.The active RNR enzyme contains two non-identical subunits called proteins R1 and R2. In mammalian cells, throughout an unperturbed cell cycle, the game of RNR is highest during S and G2 phases. This is done by de novo synthesis of this limiting R2 protein at a start of S phase, by controlled degradation of this R2 protein during mitosis.This dissertation relates to the two S phase-specific transcription of the mouse button R2 gene, and also the M phase-specific degradation of the mouse button R2 protein…
Contents: Regulation of the Expression of Mouse Ribonucleotide Reductase Small Subunit at the Levels of Transcription and Protein Degradation
Part I. Introduction
The cell cycle
Transcription
Protein degradation
Ribonucleotide reductase
Part II. Results
Transcriptional regulation of the R2 gene
Regulated protein degradation of the R2 protein
Acknowledgements
References
Papers I-IV
Regulation of the Expression of Mouse Ribonucleotide Reductase Small Subunit at the Levels of Transcription and Protein Degradation
Source: Umea University